Tue, 21 July, 2009, 17:15-18:15, Foyer
Transillumination is understood as the phenomenon of transmitting optical radiation with defined parameters by an object, which becomes the carrier of information on the characteristic size of the object. In case of biological objects the optical properties of systemic liquids and other tissues are utilized. The answer of a transilluminated object is known, but the cause can be unknown. The inverse problem is complex because more than one tissue composition may share the same resultant intensity transmittance.
The complicated object to be transilluminated may be virtually "reproduced" with the designed model based on the "sandwich" structure. This representation is performed with the division of a given object by virtual planes placed perpendicularly to transillumination direction. The structure of a specialized algorithm, which has been proposed, makes possible creating a lot of free variants of reference standards, including changes in a number of layers and their biophysical compositions. During simulations the software built with LabVIEW environment was used. Simulation of different changes in finger tissue composition allows predicting output results of occurring interactions. Experimental verification of the model structure was made with spectrophotometry.
The implemented model could be used in estimating the content and thickness of particular layers distinguished in a complex object and prediction of their transillumination efficiency. One of important purposes of presented simulation is evaluating the thickness of tissue at which discontinuity has to appear in the intensity transmittance versus this thickness. The point of transmittance discontinuity indicates a change in optical properties of a calculated layer, determining the intervals where a given transilluminated component of a tissue slab may be treated as optically thin or thick.
The presented results may be useful in computer-aided generation of reference data for evaluation of light-tissue spatial transillumination. The long-terms plans of studies are connected with the expected possibility to identify several changes in features of biological objects exposed to selective transmission in the range of wavelength including visible and near infrared radiation.
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